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SmartGene in Science - 2009

Recent journal articles and posters citing the use of SmartGene's technology are listed below. Please use the links to the left to access archived publications, organized by year.

2009

Multiplex Strategy for Multilocus Sequence Typing, fla Typing, and Genetic Determination of Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolates Collected in Switzerland. B.M. Korczak, M. Zurfluh, S. Emler, J. Kuhn-Oertli, and P. Kuhnert. Journal of Clinical Microbiology, July 2009, p. 1996-2007.

Citation: "... The rpoB sequence allowed phylogenetic differentiation between C. coli and C. jejuni, which was not possible by 16S rRNA gene analysis. An online ... platform for MLST data analysis specific to Campylobacter was implemented. This Web-based platform allowed automated allele and ST designation, as well as epidemiological analysis of data, thus streamlining and facilitating the analysis workflow. Data networking facilitates the exchange of information between collaborating centers. The described approach simplifies and improves the genotyping of Campylobacter, allowing cost- and time-efficient routine monitoring..."


DNA Sequencing for the Identification of Unusual Yeasts Encountered in a Clinical Mycology Laboratory. E.S. Slechta, J.I. Pounder, S. Cohen, and K.E. Hanson. Conference: ASM 2009, Philadelphia.

Citation: "... The SmartGene Fungal IDNS system is a web-based gene sequencing software and database program with approximately 83,000 quality controlled sequences for the ITS region, and 58,000 sequences for D1/D2, all derived from GenBank ..." (Click here to request additional information.)


Analysis of a Large Collection of non-Nocardia Aerobic Actinomycete Clinical Isolates. P.S. King, K.E. Simmon, A.Croft, and C.A. Petti. Conference: ASM 2009, Philadelphia.

Citation: "... Sequences were compared to related sequences in SmartGene ..." (Click here to request additional information.)


Identification of Mixed Infections of Mycobacterium species from Culture with 16S rRNA Gene Sequencing and RipSeq Software. G.L. Smith, V. Tesic, K.E. Simmon, and C.A. Petti. Conference: ASM 2009, Philadelphia.

Citation: "... Sequences were first analyzed with SmartGene. Electropherograms suggestive of multiple organisms were detected by observing an atypically high number of ambiguous bases (Figure 1) and in some cases an unusual susceptibility patterns ..." (Click here to request additional information.)


Direct Detection of Pathogens in Clinical Samples Using Broad Range Bacterial 16S rRNA gene PCR: a Seven Month Prospective Study. G.V. Bloemberg, P. Keller, S. Rampini, G. Dollenmaier, R. Speck, and E.C. Böttger. Conference: ASM 2009, Philadelphia.

Citation: "... homology analysis was performed using SmartGene IDNS and Genbank databases ..." (Click here to request additional information.)


A diagnostic algorithm combining phenotypic and molecular methods for identification of moulds in the clinical laboratory: report of a five year experience. D.E. Ciardo, A. Imhof, G.V. Bloemberg, E.C. Böttger. Conference: ASM 2009, Philadelphia.

Citation: "... sequence comparison with a public database (NCBI) and an in-house established yeasts & moulds ITS database (IMM validated; available through SmartGene IDNS) containing >1800 high quality sequences ..." (Click here to request additional information.)


Recognition of Novel Human Disease Associated Pathogens in the Diagnostic Microbiology. P.M. Keller, S. Rampini, A. Bächler, R. Speck, E.C. Böttger, and G.V. Bloemberg. Conference: ASM 2009, Philadelphia.

Citation: "... Homology analyses were done using SmartGene IDNS and NCBI GenBank ..." (Click here to request additional information.)


Evaluation of a COBAS Amplicor Based PCR Assay for Direct Detection and Identification of Mycobacterium spp. in Clinical Specimens. S. Peter-Getzlaff, J. Lüthy, A. Voit, G.V. Bloemberg, and E.C. Böttger, Conference: ASM 2009, Philadelphia.

Citation: "... Sequences were analyzed using SmartGene IDNS software ..." (Click here to request additional information.)