T. Lynch, D. Gregson, and DL Church. Journal of Clinical Microbiology. March 2016, Volume 54, Number 3, pages 712-717. This email address is being protected from spambots. You need JavaScript enabled to view it.

Citation: “... Compared to sequencing and identification from the SmartGene Integrated Database Network System (IDNS), Vitek MS identified 47/115 (41%) isolates to the correct species and 10 (9%) isolates to the correct genus. However, the Vitek MS was unable to provide identification for 43 (37%) isolates while 15 (13%) had discordant results. Phylogenetic analyses of the 16S rRNA sequences demonstrate high diversity in recovered Actinomyces spp. and provide additional information to compare/confirm discordant identifications between MALDI-TOF and 16S rRNA gene sequences. This study highlights the diversity of clinically relevant Actinomyces spp. and provides an important typing comparison. Based on our analysis, 16S rRNA gene sequencing should be used to rapidly identify Actinomyces spp. until MALDI-TOF databases are optimized.…A BLAST search against the SmartGene Integrated Database Network System (IDNS) for bacteria indicated the most closely related species, and the overall identity score for all isolates was ≥99.9% with 0 to 2 mismatches... In all cases, diagnosis of invasive infection was based on clinical and microbiological data from the site/source of infection, and the majority of isolates (110/115, 96%) were identified to the species level using 16S sequencing and identification with the IDNS SmartGene database. ...”