Routine drug resistance testing in HIV-1 proviral DNA, using an automated next- generation sequencing assay

EK. Alidjinou, P. Coulon, C. Hallaert, O. Robineau, A. Meybeck, T. Huleux, F. Ajana, D. Hober, and L. Bocket. Journal of Clinical Virology, 2019 Dec;121:104207. doi:10.1016/j.jcv.2019.104207. Epub 2019 Nov 2. This email address is being protected from spambots. You need JavaScript enabled to view it.

Citation: "... Sanger electropherograms were transferred to the cloud-based SmartGene IDNS® HIV-1 Module... for editing and mutations analysis... For NGS data analysis, FASTQ files were extracted from the Vela System and transferred to the SmartGene ASP-IDNS®-5 pipeline... A plausibility check was performed via pair-wise or multiple alignments between the sequences obtained with SS and NGS using the "search similar sequences" tool of the SmartGene HIV-1 Module."