Microbiology

Internal transcribed spacer region sequence analysis using SmartGene IDNS software for the identification of unusual clinical yeast isolates

ES Slechta, SL Hohmann, KE Simmon, and KE Hanson. ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA. Medical Mycology2011 November 22 (Epub ahead of print). This email address is being protected from spambots. You need JavaScript enabled to view it.

Citation: “Rapid and accurate identification of clinically important yeasts is essential given their inherent differences in antifungal susceptibility. We implemented nucleic acid sequencing for those species that could not be identified by phenotypic methods. Internal Transcribed Spacer region 1 and 2 (ITS1 and ITS2) sequences were investigated using SmartGene IDNS software … Over a 2.5-year period, 2,938 specimens were evaluated. ... Of the 169 organisms that required molecular analysis, 79% were identified to species level, 19% to genus and 2% remained unresolved. ...”